Antigen

Antigen Class

TFs and others

Antigen

SRF

Cell type

Cell type Class

Blood

Cell type

GM12878

Tissue

blood

Lineage

mesoderm

Description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Sample

source_name

GM12878

biomaterial_provider

GM12878

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

antibody antibodydescription

Rabbit polyclonal. Antibody Target: SRF

antibody targetdescription

Serum response transcription factor

antibody vendorname

Santa Cruz Biotechnology

antibody vendorid

sc-335

protocol description

a 25-cycle round of PCR and an additional 15-cycle round of PCR after gel size selection (Myers)

controlid

SL217,SL218

labexpid

SL292,SL291

labversion

PCR2x

labversion description

a 25-cycle round of PCR and an additional 15-cycle round of PCR after gel size selection (Myers)

replicate

1,2

softwareversion

MACS

antibody vendorid

sc-335

treatment

None

treatment description

No special treatment or protocol applies

protocol

PCR2x

protocol description

2-cycles of PCR (Myers)

controlid

SL217

labexpid

SL291

replicate

1

replicate description

tier 1

Sequenced DNA Library

library_name

GSM803350: HudsonAlpha_ChipSeq_GM12878_SRF_PCR2x

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

126136003

Reads aligned (%)

43.5

Duplicates removed (%)

9.2

Number of peaks

8041 (qval < 1E-05)

hg19

Number of total reads

126136003

Reads aligned (%)

42.5

Duplicates removed (%)

8.9

Number of peaks

8939 (qval < 1E-05)