Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NR3C1

Cell type

Cell type Class
Uterus
Cell type
ECC-1
Primary Tissue
Uterus
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
ECC-1
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
epithelial cell line derived from an endometrium adenocarcinoma, fetal membranes were collected from women who underwent planned cesarean delivery at term, before labor and without rupture of membranes
cell lineage
endoderm
antibody antibodydescription
Rabbit Polyclonal. Antibody Target: GR
antibody targetdescription
Glucocorticoid Receptor
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-1003
treatment description
1 h with 100 nM Dexamethasone (Myers)
controlid
SL1087,SL1035
labexpid
SL1012,SL1088
replicate
1,2
softwareversion
MACS
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-1004
treatment
DEX_100nM
treatment description
1 h with 100 nM DEX (Myers)
protocol
v041610.2
protocol description
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
controlid
SL1035
labexpid
SL1012
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803340: HudsonAlpha_ChipSeq_ECC-1_GR_DEX_100nM_v041610.2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
58054349
Reads aligned (%)
58.8
Duplicates removed (%)
15.2
Number of peaks
16630 (qval < 1E-05)

hg19

Number of total reads
58054349
Reads aligned (%)
58.4
Duplicates removed (%)
16.5
Number of peaks
16647 (qval < 1E-05)

Base call quality data from DBCLS SRA