Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD4

Cell type

Cell type Class
Others
Cell type
HSC-4
Primary Tissue
Tongue
Tissue Diagnosis
Carcinoma Squamous Cell

Attributes by original data submitter

Sample

source_name
HSC4_sg_e1_1_BRD4
cell line
HSC4
cell type
Head and neck squamous cancer cell line
treatment
CRISPRi sg-e1#1
chip antibody
BRD4 (Bethyl, A301-985A100))

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation using antibodies to enrich DNA fragments NEBNext DNA Ultra II library prep kit (E7645S)

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

hg38

Number of total reads
17559234
Reads aligned (%)
98.3
Duplicates removed (%)
28.4
Number of peaks
19085 (qval < 1E-05)

hg19

Number of total reads
17559234
Reads aligned (%)
97.7
Duplicates removed (%)
28.6
Number of peaks
18970 (qval < 1E-05)

Base call quality data from DBCLS SRA