Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K36me3

Cell type

Cell type Class
Bone
Cell type
U2OS
Tissue
bone
Lineage
mesoderm
Description
osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Attributes by original data submitter

Sample

source_name
U2OS
biomaterial_provider
ATCC
lab
USC
lab description
Farnham - University of Southern California
datatype
ChipSeq
datatype description
ChIP-Seq
cell
U2OS
cell organism
human
cell description
Osteosarcoma from the tibia of a 15 year old human female, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972. (PMID: 6081590)
cell karyotype
cancer
cell lineage
mesoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
H3K36me3B
antibody antibodydescription
rabbit polyclonal
antibody targetdescription
Histone H3 (tri-methyl K36) marks actively transcribed chromatin regions
antibody vendorname
Cell Signaling
antibody vendorid
9763S
control
UCDavis
control description
Input library was prepared at UC Davis.
control
UCDavis
control description
Input library was prepared at UC Davis.
controlid
wgEncodeEH000916
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM788076: USC_ChipSeq_U2OS_H3K36me3B_UCDavis
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg38

Number of total reads
81023712
Reads aligned (%)
87.0
Duplicates removed (%)
5.7
Number of peaks
32875 (qval < 1E-05)

hg19

Number of total reads
81023712
Reads aligned (%)
86.8
Duplicates removed (%)
6.2
Number of peaks
32996 (qval < 1E-05)

Base call quality data from DBCLS SRA