Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me1

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
ES-Bruce4
biomaterial_provider
University of California, San Diego Transgenic Core and Embryonic Stem Cell
lab
LICR-m
lab description
Ren - Ludwig Institue for Cancer Research
datatype
ChipSeq
datatype description
ChIP-Seq
line
ES-Bruce4
cell organism
mouse
biomaterial_type
mouse embryonic stem cells
cell sex
M
antibody
H3K4me1
antibody antibodydescription
rabbit polyclonal
antibody targetdescription
Histone H3 (mono methyl K4). Is associated with enhancers, and downstream of transcription starts.
antibody vendorname
Abcam
antibody vendorid
ab8895
age
E0
age description
Embryonic day 0 (stem cell)
control
std
control description
Standard input signal for most experiments.
controlid
ES-Bruce4/Input/std
labversion
12/23/09 SOLEXA2 Lane 4
replicate
1
replicate description
tier 1
softwareversion
ELAND
strain
C57BL/6
strain description
C57 black 6, the most common inbred strain of laboratory mouse

Sequenced DNA Library

library_name
GSM769009: lab_ChipSeq_ES-Bruce4_H3K4me1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
17536822
Reads aligned (%)
86.4
Duplicates removed (%)
3.7
Number of peaks
3436 (qval < 1E-05)

mm9

Number of total reads
17536822
Reads aligned (%)
86.3
Duplicates removed (%)
3.9
Number of peaks
3459 (qval < 1E-05)

Base call quality data from DBCLS SRA