Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Muscle
Cell type
HSMM
Tissue
muscle
Lineage
mesoderm
Description
skeletal muscle myoblasts

Attributes by original data submitter

Sample

source_name
HSMM
biomaterial_provider
Refer to the protocol documents for differing sources
lab
Broad
lab description
Bernstein - Broad Institute
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell organism
human
cell description
skeletal muscle myoblasts
cell lineage
mesoderm
cell sex
U
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K27ac
antibody targetdescription
Histone H3 (acetyl K27). As with H3K9ac, associated with transcriptional initiation and open chromatin structure. It remains unknown whether acetylation has can have different consequences depending on the specific lysine residue targeted. In general, though, there appears to be high redundancy. Histone acetylation is notable for susceptibility to small molecules and drugs that target histone deacetylases.
antibody vendorname
abcam
antibody vendorid
ab4729
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000119
replicate
1,2
softwareversion
ScriptureVPaperR3

Sequenced DNA Library

library_name
GSM733755: Bernstein_HSMM_H3K27ac
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg38

Number of total reads
38598478
Reads aligned (%)
65.4
Duplicates removed (%)
10.5
Number of peaks
51666 (qval < 1E-05)

hg19

Number of total reads
38598478
Reads aligned (%)
65.0
Duplicates removed (%)
11.1
Number of peaks
51550 (qval < 1E-05)

Base call quality data from DBCLS SRA