Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me2

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
HeLa-S3
biomaterial_provider
ATCC
lab
Broad
lab description
Bernstein - Broad Institute
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell organism
human
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K4me2
antibody targetdescription
Histone H3 (di methyl K4). Marks promoters and enhancers. Most CpG islands are marked by H3K4me2 in primary cells. May be associated also with poised promoters.
antibody vendorname
abcam
antibody vendorid
ab7766
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH001013
replicate
1,2
softwareversion
ScriptureVPaperR3

Sequenced DNA Library

library_name
GSM733734: Bernstein_HeLa-S3_H3K4me2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg38

Number of total reads
72963153
Reads aligned (%)
80.0
Duplicates removed (%)
11.9
Number of peaks
47043 (qval < 1E-05)

hg19

Number of total reads
72963153
Reads aligned (%)
79.4
Duplicates removed (%)
13.1
Number of peaks
46863 (qval < 1E-05)

Base call quality data from DBCLS SRA