Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Neural
Cell type
NH-A
Tissue
brain
Lineage
ectoderm
Description
astrocytes (also called Astrocy)

Attributes by original data submitter

Sample

source_name
NH-A
biomaterial_provider
Lonza
donor_id
ENCDO916IIE
biomaterial_type
primary cell
cell_type
astrocyte
lab
Broad
lab description
Bernstein - Broad Institute
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell organism
human
cell description
astrocytes (also called Astrocy)
cell karyotype
normal
cell lineage
ectoderm
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K27me3
antibody
H3K27me3
antibody targetdescription
Histone H3 (tri-methyl K27). Marks promoters that are silenced by Polycomb proteins in a given lineage; large domains are found at inactive developmental loci.
antibody vendorname
Millipore
antibody vendorid
07-449
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH001027
replicate
1,2
softwareversion
ScriptureVPaperR3

Sequenced DNA Library

library_name
GSM733729: Bernstein_NH-A_H3K27me3
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg38

Number of total reads
40436329
Reads aligned (%)
80.1
Duplicates removed (%)
8.7
Number of peaks
387 (qval < 1E-05)

hg19

Number of total reads
40436329
Reads aligned (%)
79.5
Duplicates removed (%)
9.8
Number of peaks
951 (qval < 1E-05)

Base call quality data from DBCLS SRA