Antigen

Antigen Class

Histone

Antigen

H3K4me1

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

K562

biomaterial_provider

ATCC

biomaterial_type

immortalized cell line

line

K562

lab

Broad

lab description

Bernstein - Broad Institute

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell organism

human

cell description

leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC

cell lineage

mesoderm

antibody antibodydescription

rabbit polyclonal. Antibody Target: H3K4me1

antibody targetdescription

Histone H3 (mono methyl K4). Is associated with enhancers, and downstream of transcription starts.

antibody vendorname

abcam

antibody vendorid

ab8895

treatment

None

treatment description

No special treatment or protocol applies

control

std

control description

Standard input signal for most experiments.

controlid

wgEncodeEH000052

replicate

1,2

softwareversion

ScriptureVPaperR3

Sequenced DNA Library

library_name

GSM733692: Bernstein_K562_H3K4me1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

hg38

Number of total reads

54759026

Reads aligned (%)

47.1

Duplicates removed (%)

4.5

Number of peaks

21764 (qval < 1E-05)

hg19

Number of total reads

54759026

Reads aligned (%)

46.9

Duplicates removed (%)

4.8

Number of peaks

21640 (qval < 1E-05)