SRX067422
GSM733671: Bernstein NHEK Pol2(b)

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Epidermis
Cell type
NHEK
Tissue
skin
Lineage
ectoderm
Description
epidermal keratinocytes

Attributes by original data submitter

Sample

source_name
NHEK
biomaterial_provider
Lonza
lab
Broad
lab description
Bernstein - Broad Institute
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell organism
human
cell description
epidermal keratinocytes
cell sex
U
antibody antibodydescription
mouse monoclonal, clone CTD4H8. Antibody Target: POL2B
antibody targetdescription
RNA polymerase II. Is responsible for RNA transcription. It is generally enriched at 5' gene ends, probably due to higher rate of occupancy associated with transition from initiation to elongation.
antibody vendorname
Covance
antibody vendorid
MMS-128P
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000072
replicate
1,2
softwareversion
ScriptureVPaperR3

Sequenced DNA Library

library_name
GSM733671: Bernstein_NHEK_Pol2(b)
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

Where can I get the processing logs?

Read processing pipeline log

hg38

Number of total reads
40381469
Reads aligned (%)
61.2
Duplicates removed (%)
26.9
Number of peaks
3768 (qval < 1E-05)

hg19

Number of total reads
40381469
Reads aligned (%)
60.8
Duplicates removed (%)
27.8
Number of peaks
3716 (qval < 1E-05)

Base call quality data from DBCLS SRA