Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Embryonic fibroblast

Cell type

MEF

Tissue

Embryonic Fibroblast

Lineage

primaryCells

Description

Mouse Embryonic Fibroblast

Sample

source_name

MEF

strain

C57BL/6

cell type

Fibroblasts from day 13.5 embryos immortalized with SV40-LTA

genotype

G9a f/f, ERT2-Cre

passages

Passaged 12-15 times

chip antibody

Pol II

vendor

Abcam (ab5408)

lot

722997

treatment

treated with transfection reagent only

Sequenced DNA Library

library_name

GSM610754: G9a_f_f_L2K_PolII_total

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

mm10

Number of total reads

39092349

Reads aligned (%)

80.5

Duplicates removed (%)

8.6

Number of peaks

17466 (qval < 1E-05)

mm9

Number of total reads

39092349

Reads aligned (%)

80.3

Duplicates removed (%)

8.6

Number of peaks

17513 (qval < 1E-05)