Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H4K5ac

Cell type

Cell type Class
Blood
Cell type
Macrophages
MeSH Description
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

Attributes by original data submitter

Sample

source_name
thioglycollate-elicited peritoneal macrophages
cell type
macrophage
chip antibody
H4K5ac (Millipore 07-327, Lot 1682153)
treatment
Kdo2 Lipid A for 1 hour
transgenes
none
genetic background
C57BL/6

Sequenced DNA Library

library_name
GSM579119: ThioMac-H4K5ac-Kdo2-1h-ChIP-Seq
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
16533315
Reads aligned (%)
98.9
Duplicates removed (%)
2.5
Number of peaks
5677 (qval < 1E-05)

mm9

Number of total reads
16533315
Reads aligned (%)
98.8
Duplicates removed (%)
2.6
Number of peaks
5683 (qval < 1E-05)

Base call quality data from DBCLS SRA