Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Blood

Cell type

Th1 Cells

MeSH Description

A subset of helper-inducer T-lymphocytes which synthesize and secrete INTERLEUKIN-2; INTERFERON-GAMMA; and INTERLEUKIN-12. Due to their ability to kill antigen-presenting cells and their lymphokine-mediated effector activity, Th1 cells are associated with vigorous delayed-type hypersensitivity reactions.

Sample

source_name

primary CD4+ T cells from spleen and lymph nodes

strain

C57BL6-STAT4<tm1> (STAT4KO)

cell type

in vitro polarized T helper1 cells for 7 days

passages

FACS sorted naive CD4+T cells cultured in vitro for 7 days under Th1 condition and restimulated with plate-bound CD3/28 +IL12 for 2 hrs

chip antibody

H3K27me3<Millipore #07-449>

Sequenced DNA Library

library_name

GSM550308: H3K27me3 S4KOTh1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

mm10

Number of total reads

34233436

Reads aligned (%)

31.5

Duplicates removed (%)

8.4

Number of peaks

1090 (qval < 1E-05)

mm9

Number of total reads

34233436

Reads aligned (%)

31.5

Duplicates removed (%)

8.5

Number of peaks

1116 (qval < 1E-05)