Illumina HiSeq 2000 paired end sequencing; Genome-wide occupancy of Rif1FH in mouse ESC
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
ENA checklist
ERC000011
INSDC center alias
EMBL
INSDC center name
European Molecular Biology Laboratory
INSDC first public
2015-12-24T17:01:46Z
INSDC last update
2018-03-09T00:15:50Z
INSDC status
public
SRA accession
ERS706276
Sample Name
ERS706276
alias
E-MTAB-3502:Rif1FH ESC clone FH11
broker name
ArrayExpress
cell type
mouse embryonic stem cell
organism
Mus musculus
title
Rif1FH ESC clone FH11
Sequenced DNA Library
library_name
Rif1FH ESC clone FH11
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
3 independent embryonic stem cells (ESCs) lines carrying an homozygous allele encoding for an N-terminal FLAG-HA2 Rif1 were used for ChIP seq to determine the genome-wide Ri1f occupancy. Littermate wild type cells were used as negative controls. ChIP-seq libraries were prepared with NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (NEB#E6240S/L), except that bead purification was employed (Agencourt AMPure XP beads, Beckman Coulter A63881). Size selection was performed on 2% agarose gels (E-Gel Invitrogen G6610-02). Generally 13 PCR cycles for 10ng starting material were applied. Libraries were barcoded with the NEBNext Multiplex Oligos for Illumina (Index Primers Set 1, NEB#E7335S/L).