Antigen

Antigen Class

No description

Antigen

NA

Cell type

Cell type Class

Pancreas

Cell type

Pancreas

MeSH Description

A nodular organ in the ABDOMEN that contains a mixture of ENDOCRINE GLANDS and EXOCRINE GLANDS. The small endocrine portion consists of the ISLETS OF LANGERHANS secreting a number of hormones into the blood stream. The large exocrine portion (EXOCRINE PANCREAS) is a compound acinar gland that secretes several digestive enzymes into the pancreatic ductal system that empties into the DUODENUM.

Sample

ENA-CHECKLIST

ERC000011

ENA-FIRST-PUBLIC

2014-12-23T17:01:35Z

ENA-LAST-UPDATE

2018-03-08T22:40:17Z

External Id

SAMEA3178778

INSDC center name

University of Texas Southwestern

INSDC first public

2014-12-23T17:01:35Z

INSDC last update

2018-03-08T22:40:17Z

INSDC status

public

Submitter Id

E-MTAB-3197:mouse 15 pancreas

broker name

ArrayExpress

common name

house mouse

organism part

pancreas

sample name

E-MTAB-3197:mouse 15 pancreas

scientific_name

Mus musculus

strain

C57BL/6J

Sequenced DNA Library

library_name

Ptf1a 15 ChIP

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

100 mcg of chromatin were isolated from purified nuclei of adult mouse pancreas. Aliquoting of biomaterials. Isolated chromatin was sheared to approximately 200-bp with a Bioruptor (Diagenode). Sheared chromatin was treated with 2.6 mcg of affinity-purified rabbit anti-mouse Ptf1a 7, or 10 mcg of rabbit anti-mouse Rbpjl 5 bound to Protein A/G beads (Santa Cruz, Inc.), and washed extensively. An amplified library was prepared from the bound DNA and quantified with an Agilent Bioanalyzer. 0.7 femtomoles were analyzed with an Illumina/Solexa Genome Analyzer.

Sequencing Platform

instrument_model

Illumina Genome Analyzer

mm10

Number of total reads

11571769

Reads aligned (%)

48.0

Duplicates removed (%)

58.8

Number of peaks

11589 (qval < 1E-05)

mm9

Number of total reads

11571769

Reads aligned (%)

48.0

Duplicates removed (%)

58.9

Number of peaks

11621 (qval < 1E-05)