Illumina HiSeq 1000 sequencing; SP1/2/3 and NfY cooccurency in MEFs
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
No description
Antigen
NA
Cell type
Cell type Class
Embryonic fibroblast
Cell type
MEF
Tissue
Embryonic Fibroblast
Lineage
primaryCells
Description
Mouse Embryonic Fibroblast
Attributes by original data submitter
Sample
ENA-CHECKLIST
ERC000011
ENA-FIRST-PUBLIC
2015-01-30T17:02:26Z
ENA-LAST-UPDATE
2018-03-08T20:44:35Z
External Id
SAMEA2785687
INSDC center name
Institute for Molecular Biology and Tumor Research
INSDC first public
2015-01-30T17:02:26Z
INSDC last update
2018-03-08T20:44:35Z
INSDC status
public
Submitter Id
E-MTAB-2970:Flag-Sp2FL_Sp2koMEFs
broker name
ArrayExpress
cell type
mouse embryonic fibroblast cell
common name
house mouse
genetic modification
deletion
genotype
delta Sp2
sample name
E-MTAB-2970:Flag-Sp2FL_Sp2koMEFs
scientific_name
Mus musculus
Sequenced DNA Library
library_name
Flag-Sp2FL_Sp2koMEFs
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
MEFs were kept in DMEM/Hams F-10 (1:1) (PAA/GE Healthcare) supplemented with 10% FCS (Fetal Bovine Serum Gold, PAA), 1% PenStrep (Sigma). DNA was immunoprecipitated using the respective antibody in accordance to the One-Day Chip kit (Diagenode) protocol. Four individual ChIPs were pooled and precipitated DNA was purified using Qiaquick columns (Qiagen). Five nanogram of precipitated DNA was used for library preparation using the Microplex libary preparation kit.