ChIP-Atlas: ERX531026

Antigen

Cell type Class: Liver
Cell type: Liver
MeSH Description: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

ENA-CHECKLIST: ERC000011
ENA-FIRST-PUBLIC: 2015-01-29T17:02:59Z
ENA-LAST-UPDATE: 2018-03-08T18:54:10Z
External Id: SAMEA2677849
INSDC center name: Cambridge Institute - Cancer Research UK and University of Cambridge
INSDC first public: 2015-01-29T17:02:59Z
INSDC last update: 2018-03-08T18:54:10Z
INSDC status: public
Submitter Id: E-MTAB-2633_rev:85921
broker name: ArrayExpress
common name: western European house mouse
developmental stage: adult
genotype: wild type
individual: 85921
organism part: liver
sample name: E-MTAB-2633_rev:85921
scientific_name: Mus musculus domesticus
sex: male
specimen with known storage state: fresh specimen
strain: C57BL/6J
sub_species: domesticus

library_name: DVL2-171#26
library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: For fresh specimens, hepatocytes were prepared by direct perfusion of the liver with buffered salt solution, followed by cross-linking of the diced tissue in 1% formaldehyde solution for 20 minutes, addition of 250 mM glycine and incubation for a further After homogenization of cross-linked liver tissue in a dounce tissue grinder, hepatocytes were rinsed with PBS and lysed according to published protocols {Schmidt, 2009} to solubilize DNA-protein complexes. Chromatin was fragmented to 300 bp average size 1) Immunoprecipitation. Chromatin from 0.1 g of dounced liver tissue was used for each ChIP experiment using antibodies against H3K4me3 (millipore 05-1339) and H3K27Ac (abcam ab4729) and automated 96-well protocols in an Agilent Bravo liquid handling rob