ChIP-Atlas: ERX4312250

Antigen

Cell type Class: Neural
Cell type: Thalamus
MeSH Description: Paired bodies containing mostly GRAY MATTER and forming part of the lateral wall of the THIRD VENTRICLE of the brain.

ENA first public: 2020-07-24
ENA last update: 2020-07-22
ENA-CHECKLIST: ERC000011
External Id: SAMEA7086101
INSDC center alias: Laboratory of Molecular Neurobiology Centre of New Technologies University of Warsaw
INSDC center name: Laboratory of Molecular Neurobiology Centre of New Technologies University of Warsaw
INSDC first public: 2020-07-24T04:03:48Z
INSDC last update: 2020-07-22T18:34:52Z
INSDC status: public
Submitter Id: E-MTAB-9384:KO TCF7L2 1
age: 60
broker name: ArrayExpress
common name: house mouse
developmental stage: adult
genotype: TCF7L2 knockout
individual: 15-20
organism part: thalamus
replicate: 1
sample name: E-MTAB-9384:KO TCF7L2 1
sex: male
strain: C57BL/6

library_name: KO TCF7L2 1_p
library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: Fresh thalami isolated from adult mice were cut briefly and fixed with 1% formaldehyde for 10 minutes, chromatin isolated according to Cotney and Noonan, 2015 was sonicated into 200-500 bp fragments with Covaris S220 DNA was purified with Monarch PCR & DNA Cleanup Kit (T1030, NEB) Library preparation was conducted by NGS Core Facility, Centre of New Technologies, University of Warsaw. Libraries were prepared with KAPA HyperPrep Kit and KAPA Dual-Indexed adapters (Cat. no. 7962363001, KK8722 Kapa Biosciences). Enrichment by 15 cycles of amplification was applied and final library was size-selected using Kapa Pure Beads to obtain avarage size of 350 bp. Libraries were tested for quality with High Sensitivity DNA kit (Cat. no. 5067-4626, Agilent)