ChIP-Atlas: ERX4030653

ERX4030653
Illumina HiSeq 4000 sequencing; ChIP-seq experiment in J20 hAPP mice and Pilocarpine-treated mice using deltaFosB antibody against whole-hippocampal lysate

Cell type Class: Neural
Cell type: Hippocampus
MeSH Description: A curved elevation of GRAY MATTER extending the entire length of the floor of the TEMPORAL HORN of the LATERAL VENTRICLE (see also TEMPORAL LOBE). The hippocampus proper, subiculum, and DENTATE GYRUS constitute the hippocampal formation. Sometimes authors include the ENTORHINAL CORTEX in the hippocampal formation.

library_name: 65.IN.SAL.ACTGAT_s
library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: To verify that hippocampal samples used for ΔFosB ChIP-seq came from mice with seizures and high ΔFosB expression, we first performed immunohistochemistry (Cell Signaling, D3S8R) to detect ΔFosB in one hemibrain (right side). We isolated the entire hippocampal formation from each mouse by chilling each hemibrain (left side) on an ice block, and rolling out the entire hippocampal formation using a molt elevator (Roboz, RS-8830). To perform ChIP, after removing 2% of sheared chromatin samples to use as input, chromatin fragments bound to ΔFosB were isolated using a rabbit anti-ΔFosB antibody (Cell Signaling, D3S8R), with normal rabbit IgG (Millipore 12-370) used as a negative control. All antibody concentrations were 2 μg/reaction, and samples were processed using the Magna ChIP A Kit (Millipore) for pulldown. Library preparation of ~300bp-long ChIP-seq sample fragments (NEBNext Kit, New England Biolabs) was performed by the University of Pennsylvania Next-Generation Sequencing Core prior to Illumina sequencing.