Illumina HiSeq 2500 sequencing; MAFB ChIPseq in Human EndoCBH2 cells
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
No description
Antigen
NA
Cell type
Cell type Class
Pancreas
Cell type
EndoC-BH2
NA
NA
Attributes by original data submitter
Sample
ENA first public
2020-02-01
ENA last update
2019-12-18
ENA-CHECKLIST
ERC000011
External Id
SAMEA6428046
INSDC center alias
VANDERBILT UNIVERSITY
INSDC center name
VANDERBILT UNIVERSITY
INSDC first public
2020-02-01T04:03:05Z
INSDC last update
2019-12-18T14:22:09Z
INSDC status
public
Submitter Id
E-MTAB-8612:MAFB sample 2
broker name
ArrayExpress
cell line
EndoC-betaH2
cell type
type B pancreatic cell
common name
human
developmental stage
embryo
genotype
wild type genotype
organism part
pancreas
sample name
E-MTAB-8612:MAFB sample 2
Sequenced DNA Library
library_name
MAFB sample 2_s
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was prepared from EndoC-βH2 cells by cross-linking cells for 10 minutes with 1% formaldehyde. EndoC-βH2 cells were grown in DMEM containing 5.6 mM glucose, 2% BSA, 50 μM 2-mercaptoethanol, 10 mM nicotinamide, 5.5 μg/mL transferrin, 6.7 ng/mL selenite, 100 units/mL penicillin, and 100 units/mL streptomycin (Scharfmann et al., 2014). Cells were collected and lysed in SDS buffer (Millipore Chromatin Immunoprecipitation Assay Kit. Cat#17-295), and chromatin was sonicated using a Diagenode Bioruptor. Standard DNA sequencing library for sequencing on Illumina sequencing platforms.