ChIP-Atlas: ERX3449686

ERX3449686
HiSeq X Ten sequencing; The H3K4me3 pattern of mouse renal cortex tissues treated with dexmedetomidine against lipopolysaccharide-induced sepsis

Cell type Class: Kidney
Cell type: Kidney Glomerulus
MeSH Description: A cluster of convoluted capillaries beginning at each nephric tubule in the kidney and held together by connective tissue.

ENA first public: 2020-07-03
ENA last update: 2019-07-09
ENA-CHECKLIST: ERC000011
External Id: SAMEA5765089
INSDC center alias: Department of Critical Care Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, 250000, China.
INSDC center name: Department of Critical Care Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, 250000, China.
INSDC first public: 2020-07-03T04:03:13Z
INSDC last update: 2019-07-09T17:47:39Z
INSDC status: public
Submitter Id: E-MTAB-8133:AKI+D_H3K4me3_2
age: 6 to 8
broker name: ArrayExpress
common name: house mouse
developmental stage: adult
genotype: wild type genotype
individual: 1
organism part: renal glomerulus
sample name: E-MTAB-8133:AKI+D_H3K4me3_2
sex: male
strain: C57BL/6

library_name: AKI+D_H3K4me3_2_s
library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: Mouse kidney tissues were removed capsule, and cut into small pieces in cold PBS, and added RIPA buffer (0.5% SDS) on ice for 30 min, then centrifuged at 13000 rpm for 15 min. The supernatant was harvested and treated by sonication to break genomic DNA into 200-500 bp. 10% were stored as input and the rest of supernatant was quantified the equal DNA concentration, then incubated with 1 μg IP grade antibodies of H3K4me3 (Activemotif, USA) 4 °C overnight. Protein-A beads were incubated next day at 4 °C for target protein pull down. template DNA was repaired to 3'-dA overhang and added the ligated adapter. The DNA library was eliminated the unligated adapters and selected the appropriate size for sequence using an Illumina Hiseq 2000 platform.