Illumina HiSeq 2000 sequencing; Regulatory genomics of human embryonic pancreatic progenitors
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
No description
Antigen
NA
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC H9
NA
NA
Attributes by original data submitter
Sample
ENA first public
2013-11-15
ENA last update
2018-03-08
ENA-CHECKLIST
ERC000011
External Id
SAMEA2229609
INSDC center alias
Hospital Clinic de Barcelona
INSDC center name
Hospital Clinic de Barcelona
INSDC first public
2013-11-15T17:01:11Z
INSDC last update
2018-03-08T16:51:40Z
INSDC status
public
Submitter Id
E-MTAB-1990:in vitro human pancreatic progenitor cells (PP2)
broker name
ArrayExpress
cell line
H9
cell type
embryonic stem cell
common name
human
sample name
E-MTAB-1990:in vitro human pancreatic progenitor cells (PP2)
Sequenced DNA Library
library_name
PP2_3_G6
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The differentiation protocol is described in Cho, C.H. et al. Inhibition of activin/nodal signalling is necessary for pancreatic differentiation of human pluripotent stem cells. Diabetologia 55, 3284-3295 (2012) In vitro human pancreatic progenitor cells were fixed, sonicated and processed for ChIP as described (van Arensbergen, J. et al. Genome Res 20:722, 2010) using 1-1.5ug of the following antibodies: rabbit anti-H3K4me1 (ab-8895), goat anti-Pdx1 (Chris Wright, BCBC AB2027), goat anti-FOXA2 (sc-6554), rabbit anti-GATA6 (sc-9055X), rabbit anti-HNF1B(sc-22840-X), rabbit anti-ONECUT1 (sc-13050). The immunoprecipitated DNA was modified for sequencing following the Illumina sequencing following the manufacturer protocol (Preparing Sample for ChIP Sequencing of DNA (11257047 Rev A)).