Effect of FOXA1 over-expression upon AR binding in AR-driven cancers
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
No description
Antigen
NA
Cell type
Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
ENA first public
2014-01-14
ENA last update
2018-03-08
External Id
SAMEA2157287
INSDC center alias
Cancer Research UK Cambridge Institute
INSDC center name
Cancer Research UK Cambridge Institute
INSDC first public
2014-01-14T17:00:44Z
INSDC last update
2018-03-08T16:35:52Z
INSDC status
public
Submitter Id
E-MTAB-1749:LNCaP
broker name
ArrayExpress
cell line
LNCaP
cell type
epithelial
cell type
prostate
common name
human
sample name
E-MTAB-1749:LNCaP
sex
male
specimen with known storage state
fresh specimen
Sequenced DNA Library
library_name
jc965
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The LNCaP prostate cancer cell line and the ER-AR+ molecular apocrine breast cancer cell line, MDA-MB-453, were obtained from American Type Culture Collection (ATCC). Cells were grown in RPMI or DMEM media respectively, both supplemented with 10% Fetal Bovine Serum, 2 mM L-glutamine, 50U/ml penicillin and 50ug/ml streptomycin in 37oC incubator with 5% CO2. Cells were transfected with 3ug pcDNA3.1_GFP plasmid DNA using Lipofectamine2000 (Invitrogen). Cells were harvested 48hours after treatment. For all experiments, 1x10^8 cells were cross-linked with 1% formaldehyde as previously described (Schmidt et al, Methods 48(3) July 2009 doi:10.1016/j.ymeth.2009.03.001). ChIP-seq assays were performed as previously described (Schmidt 2009). Protein-bound DNA was immunoprecipitated with an antibody against androgen receptor (AR) (Santa Cruz, sc-816). Immunoprecipitated DNA was end-repaired, A-tailed and single-end Illumina sequencing adapter ligated before 18 cycles of PCR amplification. A 2% agarose gel was used to select 200-300 bp DNA fragments.