Illumina HiSeq 4000 paired end sequencing; ChIP-seq of Zic3 in ES-E14Tg2a embryonic stem cells after 2 days of differentiation to epiblast-like cells
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
No description
Antigen
NA
Cell type
Cell type Class
Pluripotent stem cell
Cell type
EpiLC
NA
NA
Attributes by original data submitter
Sample
ENA first public
2019-01-31
ENA last update
2018-09-14
ENA-CHECKLIST
ERC000011
External Id
SAMEA4896486
INSDC center alias
The University of Manchester
INSDC center name
The University of Manchester
INSDC first public
2019-01-31T17:02:06Z
INSDC last update
2018-09-14T10:33:38Z
INSDC status
public
Submitter Id
E-MTAB-7208:Sample 5
broker name
ArrayExpress
cell line
ES-E14TG2a
cell type
epiblast-like cell
common name
house mouse
disease
normal
genotype
Rex1GFPd2
organism part
inner cell mass
progenitor cell type
embryonic stem cell
sample name
E-MTAB-7208:Sample 5
Sequenced DNA Library
library_name
Sample 5_p
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIPmentaion experiments were performed using ZIC3 (Abcam, ab222124) antibody in the Rex1GFPd2 mouse embryonic stem cell line (ESC; parental line; E14Tg2a) and d1- and d2-EpiLC differentiated states. Cells were grown in 12ng/ml bFGF, 20ng/ml activin A, 1% KOSR on 5ug/ml bovine plasma fibronectin-coated dishes. ChIPmentation samples and libraries were generated as described previously (Schmidl et al. 2015 https://doi.org/10.1038/nmeth.3542) ChIPmentation Nextera XT DNA library preparation kit