NextSeq 500 sequencing; FloChIP's sequential chip data
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
No description
Antigen
NA
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
ENA first public
2019-01-10
ENA last update
2018-09-05
ENA-CHECKLIST
ERC000011
External Id
SAMEA4886170
INSDC center alias
Ecole Polytechnique Federale de Lausanne
INSDC center name
Ecole Polytechnique Federale de Lausanne
INSDC first public
2019-01-10T17:02:41Z
INSDC last update
2018-09-05T14:31:25Z
INSDC status
public
Submitter Id
E-MTAB-7186:Sample 1
broker name
ArrayExpress
cell line
ES-E14TG2a
cell type
embryonic stem cell
common name
house mouse
disease
normal
genotype
wild type genotype
organism part
inner cell mass
sample name
E-MTAB-7186:Sample 1
Sequenced DNA Library
library_name
Sample 1_s
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin from mouse ES-E14TG2a cells was immunoprecipitated by FloChIP technique - a novel microfluidic immunoprecipitation system, the details of which will be published in due course - using the following Abcam antibodies: H3k27ac (ab47290; H3k4me3 (ab8580), H3k4me1 (ab8895), H3k9me3 (ab8898), H3k27me3 (ab6147) PCR purification Nextera