EUROPEAN MOLECUALR BIOLOGY LABORATORY, GENOME BIOLOGY UNIT
INSDC center name
EUROPEAN MOLECUALR BIOLOGY LABORATORY, GENOME BIOLOGY UNIT
INSDC first public
2018-11-19T17:03:21Z
INSDC last update
2018-09-04T15:12:18Z
INSDC status
public
IndividualGeneticCharacteristics
Setd5 delta-SET Homozygous Knock Out
Submitter Id
embase-sample-28007:ChIP_Input_G6
cell line
ESC 129-B13
common name
house mouse
geneticmodification
gene knock out
sample name
embase-sample-28007:ChIP_Input_G6
sampletype
frozen_sample
sex
male
Sequenced DNA Library
library_name
ChIP_Input_G6
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Protocols : Extraction Protocol: Elution of the IP chromatin from the beads was performed in elution buffer (1% SDS) for 30 min at 65C. Eluted chromatin and ChIP inputs were reverse cross linked and proteins were digested with proteinase K overnight at 65C. DNA was purified using a PCR purification kit (QIAgen). Libraries were prepared with NEBNext Ultra II kit. Library Protocol: For ChIP Input 100 ng of prepared MNase-digested mildly sonicated DNA was used for library preparation. For ChIP, entire IP-eluate was used for library preparation. Sequencing libraries were prepared using DNA Ultra II library preparation kit (NEB) according to the manufacturer instructions. Number of Amplification cycles was adjusted depending on ChIP. Samples were barcoded, pooled and sequenced on Illumina HiSeq2000 Sequencer (50 bp single-end mode).