ChIP-Atlas: ERX2746349

ERX2746349
NextSeq 500 sequencing; Androgen Promotes Differentiation of Promyelocytic Leukemia Zinc Finger (PLZF)+ Spermatogonia pool via Indirect Regulatory Pattern

Cell type Class: Gonad
Cell type: Testis
MeSH Description: The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.

ENA first public: 2018-08-20
ENA last update: 2018-08-10
ENA-CHECKLIST: ERC000011
External Id: SAMEA4825582
INSDC center alias: School of biomedical engineering,Shanghai Jiao Tong University
INSDC center name: School of biomedical engineering,Shanghai Jiao Tong University
INSDC first public: 2018-08-20T17:02:03Z
INSDC last update: 2018-08-10T10:27:52Z
INSDC status: public
Submitter Id: E-MTAB-7129:ChIP-seq-wt1-SSC
age: 6
broker name: ArrayExpress
cell type: Sertoli cell
common name: house mouse
genotype: wild type genotype
organism part: testis
sample name: E-MTAB-7129:ChIP-seq-wt1-SSC
sex: male
strain: SSC

library_name: ChIP-seq-wt1-SSC_s
library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: Sertoli cells were isolated from testes of 6 dpp mice and maintained in DMEM/F12 media supplemented with 2.5%FBS, 5%horse serum, 2mML-Gln, 100U/mLpenicillin, 1uMsodium pyruvate Sertoli cells lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Cells were cross-linked with 1% formaldehyde for 10 min at room temperature and formaldehyde was then inactivated by the addition of 125 mM glycine for 5min. Sonicated DNA fragments with 100–300 bp were pre-cleared and then immunoprecipitated with Protein A+G Magnetic beads coupled with Anti-Androgen Receptor antibody (06–680, Millipore).