NextSeq 500 sequencing; ChIP-exo data for 21 transcription factors of yeast metabolic gene regulation in four different chemostat conditions
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
No description
Antigen
NA
Cell type
Cell type Class
Unclassified
Cell type
Unclassified
NA
NA
Attributes by original data submitter
Sample
ENA first public
2019-03-20
ENA last update
2018-04-18
ENA-CHECKLIST
ERC000011
External Id
SAMEA4590508
INSDC center alias
CHALMERS
INSDC center name
Chalmers University of Technology
INSDC first public
2019-03-20T17:02:21Z
INSDC last update
2018-04-18T13:35:45Z
INSDC status
public
Submitter Id
E-MTAB-6673:Stb5 Ana 1
broker name
ArrayExpress
common name
baker's yeast
genotype
9xMyc-STB5
growth condition
synthetic media in chemostat
sample name
E-MTAB-6673:Stb5 Ana 1
strain
CEN.PK-113-5D KiURA3
Sequenced DNA Library
library_name
Stb5 Ana 1_s
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
From stable chemostats, 100OD were collected for each biological replicate and added formaldehyde for crosslinking protein-DNA Chemostats with synthetic media Synthetic media designed to limit growth by a specific nutrient. Different environmental stresses are described as follows: low nitrogen: Minimal media with 6% glucose and 1 g/L NH4SO4. Pressurized air supplied. low ethanol: Minimal media with 0.5% ethanol and 5 g/L NH4SO4. Pressurized air supplied. anaerobic conditions; low glucose: Minimal media with 1% glucose and 5 g/L NH4SO4. N2 supplied. low respiratory glucose: Minimal media with 0.75% glucose and 5 g/L NH4SO4. Pressurized air supplied Yeast cells lysed by glass beads and sonicated. ChIP-exo protocol followed for capturing and extracting specific DNA Library constructed during the ChIP-exo protocol as described here: ChIP-exo: A Method to Identify Genomic Location of DNA-binding proteins at Near Single Nucleotide Accuracy doi: 10.1002/0471142727.mb2124s100