ChIP-Atlas: ERX2190888

Antigen

Cell type Class: Liver
Cell type: Liver
MeSH Description: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

ENA first public: 2018-04-26
ENA last update: 2017-09-22
ENA-CHECKLIST: ERC000011
External Id: SAMEA104313374
INSDC center alias: Cell Signal Unit, Okinawa Institute of Science and Technology Graduate University
INSDC center name: Cell Signal Unit, Okinawa Institute of Science and Technology Graduate University
INSDC first public: 2018-04-26T17:02:33Z
INSDC last update: 2017-09-22T10:52:20Z
INSDC status: public
Submitter Id: E-MTAB-5887:ChIP_Cnot1_control_liver_2
age: 10
broker name: ArrayExpress
common name: house mouse
genotype: Cnot1 flox/flox
organism part: liver
phenotype: wild type phenotype
sample name: E-MTAB-5887:ChIP_Cnot1_control_liver_2
sex: male
strain: C57BL/6J

library_name: ChIP_Cnot1_control_liver_2_p
library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: Mice were maintained on a 12 hr light / dark cycle in a temperature-controlled (22ºC) barrier facility with free access to water and a normal diet (NCD, CA-1, CLEA Japan, Inc.). Control (Cnot1fl/fl) and Cnot1 LKO mice placed on tamoxifen-containing food at 6 weeks of age for 2 weeks. Lysate from liver was subjected to ChIP assay with Histone H3 (tri methyl K4) (H3K4me3) antibody (ab8580; abcam) using SimpleChIP Enzyatic Chromatin IP Kit (#9003; Cell Signaling Technology) with modification. Briefly, after micrococcal nuclease treatment, cross-linked chromatin was incubated with anti-H3K4me3 antibody at 4ºC for 24 hr. The immune complex was washed, and DNA was eluted and de-cross-linked. Libraries for DNA sequence were prepared from DNA isolated by ChIP assays with a KAPA Hyper Prep Kit (Illumina), according to the manufacturer's protocol.