Illumina HiSeq 2500 sequencing; ChIP-seq of SATB1-bound regions in human ESC-derived dopaminergic neurons (60 days of differentiation) treated with 6OHDA
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
No description
Antigen
NA
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC derived neural cells
NA
NA
Attributes by original data submitter
Sample
ENA first public
2018-07-22
ENA last update
2017-08-15
ENA-CHECKLIST
ERC000011
External Id
SAMEA104215616
INSDC center alias
The Rockefeller University Laboratory of Molecular and Cellular Neuroscience
INSDC center name
The Rockefeller University Laboratory of Molecular and Cellular Neuroscience
INSDC first public
2018-07-22T17:01:55Z
INSDC last update
2017-08-15T15:26:59Z
INSDC status
public
Submitter Id
E-MTAB-5965:MR35
broker name
ArrayExpress
cell line
H9
cell type
dopaminergic neuron
common name
human
genotype
NURR1::GFP
progenitor cell type
embryonic stem cell
sample name
E-MTAB-5965:MR35
Sequenced DNA Library
library_name
MR35_s
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The hESCs H9 (WA-09)-NURR1-GFP reporter line was maintained using E8-essential medium (Fisher Scientific) without feeder on VTA-N (Fisher Scientific) and passaged every 4-5 days by EDTA. Midbrain dopamine (mDA) differentiation from hESC was done with an optimized protocol from previously published by our group. mock (solvent) or 50uM 6OHDA treatment for 16h ChIP was performed and DNA was purified with a magnetic bead-based purification method according to the manufacturer's protocol (MAGnify Chromatin Immunoprecipitation System-Kit, LifeTechnologies). sheared DNA was used to generate ChIP-sequencing libraries by employing Ovation Ultralow System V2 Kit (NuGEN).