ChIP-Atlas: ERX2070513

Antigen

Cell type Class: Liver
Cell type: Liver
MeSH Description: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

ENA first public: 2017-06-29
ENA last update: 2017-06-19
ENA-CHECKLIST: ERC000011
External Id: SAMEA104128244
INSDC center alias: Helmholtz Zentrum Munchen German Research Center for Environmental Health Institute for Diabetes and Cancer
INSDC center name: Helmholtz Zentrum Munchen German Research Center for Environmental Health Institute for Diabetes and Cancer
INSDC first public: 2017-06-29T17:01:47Z
INSDC last update: 2017-06-19T13:42:53Z
INSDC status: public
Submitter Id: E-MTAB-5831:Gabpa_2
age: 12
broker name: ArrayExpress
common name: house mouse
genotype: wild type genotype
organism part: liver
sample name: E-MTAB-5831:Gabpa_2
sex: male
strain: C57BL/6J

library_name: Gabpa_2_s
library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: Male 12wk old C57Bl/6J mice were obtained from Charles River Laboratories (Brussels, Belgium) and maintained on a 12 h light-dark cycle with regular, unrestricted access to water. Pulverized liver tissue from 12 wks old male C57Bl/6J mice was fixed with 1 % formaldehyde in PBS extracts were homogenized by douncing in lysis buffer and chromatin fragmentation and ChIP assays were performed as described (Canettieri et al., 2003) using antibodies specific for GAbpα or unspecific IgG.Cross-linking was reversed overnight at 65°C, and DNA isolated using MinElute PCR Purification Kit. Enriched DNA from ChIP and Input DNA fragments were end-repaired, extended with an 'A' base on the 3end, ligated with indexed paired-end adaptors using the Bravo Platform, size-selected after 4 cycles of PCR with AMPure XP beads and amplified by PCR for 10 cycles more. Libraries were prepared from approximately 50 pg of ChIP DNA using the ThruPlex-FD Prep Kit