Antigen

Antigen Class

No description

Antigen

NA

Cell type

Cell type Class

Blood

Cell type

Erythrocytes

MeSH Description

Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.

Sample

ENA first public

2013-03-26

ENA last update

2018-03-08

External Id

SAMEA1713728

INSDC center alias

BSRC Alexander Fleming, Division of Molecular Oncology

INSDC center name

BSRC Alexander Fleming, Division of Molecular Oncology

INSDC first public

2013-03-26T17:02:31Z

INSDC last update

2018-03-08T16:16:40Z

INSDC status

public

Submitter Id

E-MTAB-1504:Ter119_pos_E12.5_Fetal_Liver_Cells

age

12.5 day

broker name

ArrayExpress

cell type

Erythroid

common name

house mouse

developmental stage

Ter119+ differentiated erythrocytes

organism part

Fetal liver

sample name

E-MTAB-1504:Ter119_pos_E12.5_Fetal_Liver_Cells

strain

C57BL/6

Sequenced DNA Library

library_name

GATA1_Ter119_pos_R1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Fetal liver cells from day E12.5 C57/BL6 mouse embryos expanded for 3 days in serum free medium (von Lindern et al, 2001) Fractionation of Ter119- and Ter119+ cells with Miltenyi beads (Schuh et al, 2005) Formaldehyde-crosslinked chromatin from 10^7 Ter119- or Ter119+ fetal liver cells was prepared as previously described (Schuh et al, 2005)

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

mm10

Number of total reads

12600338

Reads aligned (%)

94.8

Duplicates removed (%)

81.5

Number of peaks

1643 (qval < 1E-05)

mm9

Number of total reads

12600338

Reads aligned (%)

94.5

Duplicates removed (%)

81.8

Number of peaks

1635 (qval < 1E-05)