Antigen

Antigen Class

No description

Antigen

NA

Cell type

Cell type Class

Blood

Cell type

Erythroblasts

MeSH Description

Immature, nucleated ERYTHROCYTES occupying the stage of ERYTHROPOIESIS that follows formation of ERYTHROID PRECURSOR CELLS and precedes formation of RETICULOCYTES. The normal series is called normoblasts. Cells called MEGALOBLASTS are a pathologic series of erythroblasts.

Sample

ENA first public

2013-03-26

ENA last update

2018-03-08

External Id

SAMEA1713724

INSDC center alias

BSRC Alexander Fleming, Division of Molecular Oncology

INSDC center name

BSRC Alexander Fleming, Division of Molecular Oncology

INSDC first public

2013-03-26T17:02:31Z

INSDC last update

2018-03-08T16:16:40Z

INSDC status

public

Submitter Id

E-MTAB-1504:Ter119_neg_E12.5_Fetal_Liver_Cells

age

12.5 day

broker name

ArrayExpress

cell type

Erythroid

common name

house mouse

developmental stage

Ter119- proerythroblasts

organism part

Fetal liver

sample name

E-MTAB-1504:Ter119_neg_E12.5_Fetal_Liver_Cells

strain

C57BL/6

Sequenced DNA Library

library_name

GATA1_Ter119_neg_R1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Fetal liver cells from day E12.5 C57/BL6 mouse embryos expanded for 3 days in serum free medium (von Lindern et al, 2001) Fractionation of Ter119- and Ter119+ cells with Miltenyi beads (Schuh et al, 2005) Formaldehyde-crosslinked chromatin from 10^7 Ter119- or Ter119+ fetal liver cells was prepared as previously described (Schuh et al, 2005)

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

mm10

Number of total reads

14608407

Reads aligned (%)

72.4

Duplicates removed (%)

13.7

Number of peaks

21686 (qval < 1E-05)

mm9

Number of total reads

14608407

Reads aligned (%)

72.2

Duplicates removed (%)

13.9

Number of peaks

21644 (qval < 1E-05)