Illumina HiSeq 2000 sequencing; ChIP-seq for Zeb1 in Human Neural Stem Cells and Glioblastoma Cancer Stem-like Cells
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
No description
Antigen
NA
Cell type
Cell type Class
Neural
Cell type
Glioblastoma multiforme
NA
NA
Attributes by original data submitter
Sample
ENA first public
2018-04-24
ENA last update
2017-03-06
ENA-CHECKLIST
ERC000011
External Id
SAMEA103891181
INSDC center alias
Instituto Gulbenkian de Ciencia
INSDC center name
Instituto Gulbenkian de Ciencia
INSDC first public
2018-04-24T17:03:46Z
INSDC last update
2017-03-06T12:10:54Z
INSDC status
public
Submitter Id
E-MTAB-5541:Zeb1_NCH421k
broker name
ArrayExpress
cell line
D4 glioblastoma derived primary cell line
cell type
cancer stem-like cell
common name
human
disease
glioblastoma multiforme
genotype
NCH421k
growth condition
media
sample name
E-MTAB-5541:Zeb1_NCH421k
tumor grading
IV
Sequenced DNA Library
library_name
Zeb1_NCH421k_s
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were cultured as previously described (Conti et al. 2005), using laminin as a media supplement (20ug/mL), and plated at a density of 28,000/cm2 Cells were fixed sequentially with di(N-succimidyl) glutarate and 1% formaldehyde in phosphate buffered saline and then lysed, sonicated and immunoprecipitated, as previously described (Castro et al., 2011), with an anti-ZEB1 antibody DNA libraries were prepared from 10ng of immunoprecipitated DNA according to the standard Illumina ChIP-seq protocol.