Illumina HiSeq 2500 paired end sequencing; ChIP-Seq (H3K4me3, H3K27me3) of steady-state splenic classical dendritic cells from wild type, germ-free and IFNAR knockout mice
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
No description
Antigen
NA
Cell type
Cell type Class
Spleen
Cell type
Spleen
MeSH Description
An encapsulated lymphatic organ through which venous blood filters.
Attributes by original data submitter
Sample
ENA first public
2018-06-30
ENA last update
2016-11-15
ENA-CHECKLIST
ERC000011
External Id
SAMEA4551893
INSDC center alias
Mainz University Medical Center Institute for Medical Microbiology and Hygiene
INSDC center name
Mainz University Medical Center Institute for Medical Microbiology and Hygiene
INSDC first public
2018-06-30T17:02:37Z
INSDC last update
2016-11-15T17:14:10Z
INSDC status
public
Submitter Id
E-MTAB-5234:WT2Input
age
8
broker name
ArrayExpress
common name
house mouse
genotype
wild type genotype
organism part
spleen
sample name
E-MTAB-5234:WT2Input
sex
female
strain
C57BL/6
Sequenced DNA Library
library_name
WT2Input_p
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Splenocytes were isolated and sorted for CD3 CD19 CD45 CD11c MHC-II to high purity. Fixed chromatin from isolated cells was sheared and precipitated for H3K4me3 and H3K27me3 Libraries were prepared by the Deep Sequencing Unit at the MPI in Freiburg, Germany