Illumina Genome Analyzer II sequencing; Genome-wide profiling of Glucocorticoid receptor binding in A549, Nalm-6, MEF WT and MEF GCN5/PCAF double knockout (ChIP-seq)
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
No description
Antigen
NA
Cell type
Cell type Class
Blood
Cell type
NALM-6
Primary Tissue
Blood
Tissue Diagnosis
Leukemia
Attributes by original data submitter
Sample
ENA first public
2016-11-10
ENA last update
2016-09-22
ENA-CHECKLIST
ERC000011
External Id
SAMEA4462127
INSDC center alias
Institut de Biologie de l'Ecole Normale Superieure
INSDC center name
Institut de Biologie de l'Ecole Normale Superieure
INSDC first public
2016-11-10T17:08:24Z
INSDC last update
2016-09-22T16:57:16Z
INSDC status
public
Submitter Id
E-MTAB-5113:Nalm-6_input
broker name
ArrayExpress
cell line
NALM6
common name
human
genotype
wild type genotype
sample name
E-MTAB-5113:Nalm-6_input
Sequenced DNA Library
library_name
Nalm-6_input_s
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were grown in DMEM supplemented with 10% FBS. Approximately 10 million cells were treated with 0.01% ethanol vehicle or 1 uM dexamethasone for 1.5 h. Chromatin was sheared with a Bio-ruptor water bath sonicator (Diagenode) to produce fragments of 100-200 bp. Protein-G coupled magnetic beads (Active- Motif) were preincubated for 1 h with GR-antibody (N499) before chromatin was added and incubated for an additional 2-4 h while rotating at 4C. Subsequently beads were washed three times with 10 mM TrisaHCl pH 8.0, 1 mM EDTA, 500 mM NaCl, 5% (vol/vol) glycerol, 0.1% sodium deoxycholate, 0.1% SDS, 1% Triton X-100, 0.5 mg/uL BSA, followed by three additional washes with 20 mM Tris, pH 8.0, 1 mM EDTA, 250 mM LiCl, 0.5% Nonidet P-40, and 0.5% sodium deoxycholate.