Sample information curated by ChIP-Atlas

Antigen

Antigen Class
No description
Antigen
NA

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

ENA first public
2018-07-30
ENA last update
2016-08-09
ENA-CHECKLIST
ERC000011
External Id
SAMEA4376684
INSDC center alias
Institute of Biochemistry University Stuttgart
INSDC center name
Institute of Biochemistry University Stuttgart
INSDC first public
2018-07-30T17:02:43Z
INSDC last update
2016-08-09T16:45:59Z
INSDC status
public
Submitter Id
E-MTAB-4982:3TD replicate 1
broker name
ArrayExpress
cell line
E14
cell type
embryonic stem cell
common name
house mouse
sample name
E-MTAB-4982:3TD replicate 1

Sequenced DNA Library

library_name
3TD replicate 1_s
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Mouse embryonic stem cells ESCs line E14 (129/Ola) were cultured without feeders in the presence of LIF in DMEM containing 15% fetal calf serum. Chromatin IP kit (Active Motif)+ Standard Illumina library preparation

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
49834480
Reads aligned (%)
98.0
Duplicates removed (%)
7.8
Number of peaks
385 (qval < 1E-05)

mm9

Number of total reads
49834480
Reads aligned (%)
97.9
Duplicates removed (%)
7.9
Number of peaks
369 (qval < 1E-05)

Base call quality data from DBCLS SRA