ChIP-Atlas: ERX161939

Antigen

Cell type Class: Gonad
Cell type: Testis
MeSH Description: The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.

ENA-CHECKLIST: ERC000011
ENA-FIRST-PUBLIC: 2013-07-12T17:00:49Z
ENA-LAST-UPDATE: 2018-03-08T16:37:09Z
External Id: SAMEA2156575
INSDC center name: Cancer Research UK, Cambridge Research Institute, Robinson Way, Cambridge, UK
INSDC first public: 2013-07-12T17:00:49Z
INSDC last update: 2018-03-08T16:37:09Z
INSDC status: public
Submitter Id: E-MTAB-1104:tc152038_testes
age: 2 to 6 month
broker name: ArrayExpress
common name: house mouse
developmental stage: adult
genotype: Tc1_with_hschr21
individual: tc152038
organism part: testes
sample name: E-MTAB-1104:tc152038_testes
scientific_name: Mus musculus
sex: male

library_name: do1853_PolIII_testes_1900_tc1_CRI01
library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: doi:10.1016/j.ymeth.2009.03.001; Crosslinked animal livers were dounce homogenized into a single cell suspension and rinsed with ice cold PBS. Each pellet of cross-linked material was resuspended in 10 ml of LB1 (50 mM Hepes-KOH, pH 7.5; 140 mM NaCl; 1mM EDTA; 10% Glycerol; 0.5% NP-40 or Igepal CA-630; 0.25% Triton X-100) and pelleted again by centrifugation at 2,000 x rcf for 4 minutes at 4 C. Pellets were then rinsed in 10 ml of LB2 (10 mM Tris-HCL, pH8.0; 200 mM NaCl; 1 mM EDTA; 0.5 mM EGTA) and pelleted again by centrifugation at 2,000 x rcf for 4 minutes at 4 C. The nuclei preparation was resuspended in 3 ml LB3 (10 mM Tris-HCl, pH 8; 100 mM NaCl; 1 mM EDTA; 0.5 mM EGTA; 0.1% Na-Deoxycholate; 0.5% N-lauroylsarcosine). Chromatin in the nuclei fraction was fragmented to an average length of 300 bp by sonication. After sonication 300 l of 10% Triton X-100 was added to the 3 ml of sonicated lysate and debris was removed by centrifugation at 20,000 x rcf for 10 minutes at 4 C.