Illumina HiSeq 2500 sequencing; A cytosolic Ezh1 isoform modulates PRC2-Ezh1 repressive function as adaptive epigenetic response in post-mitotic cells
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
No description
Antigen
NA
Cell type
Cell type Class
Muscle
Cell type
C2C12
Primary Tissue
Skeletal Muscle
Tissue Diagnosis
NOS
Attributes by original data submitter
Sample
ENA first public
2017-03-01
ENA last update
2016-06-16
ENA-CHECKLIST
ERC000011
External Id
SAMEA4036476
INSDC center alias
INGM Istituto Nazionale di Genetica Molecolare \Romeo ed Enrica Invernizzi\
INSDC center name
INGM Istituto Nazionale di Genetica Molecolare \Romeo ed Enrica Invernizzi\
INSDC first public
2017-03-01T17:07:12Z
INSDC last update
2016-06-16T22:02:52Z
INSDC status
public
Submitter Id
E-MTAB-4794:Input_H2O2
broker name
ArrayExpress
cell line
C2C12
common name
house mouse
sample name
E-MTAB-4794:Input_H2O2
stimulus
Hydrogen peroxide
Sequenced DNA Library
library_name
Input_H2O2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Myotubes were grown in adherent condition, they are fixed in Formaldehyde 1% solution, quenched in Glycine, washed in PBS and then collected with scrapers. Cells are opportunely lised and the chromatin is then sonicated to final size of about 300bp. Chromatin was immunoprecipitated with the described antibody Confluent myoblasts are differentiated in DMEM containing 2% Horse Serum, at 37°C in 5% of CO2 following standard protocols Myotubes day 1 were treated with 100 uM of H2O2 for 24 hours Illumina prep library