Antigen

Antigen Class

No description

Antigen

NA

Cell type

Cell type Class

Gonad

Cell type

Testis

MeSH Description

The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.

Sample

ENA first public

2014-11-20

ENA last update

2018-03-08

External Id

SAMEA2024558

INSDC center alias

EMBL-EBI

INSDC center name

EMBL-EBI

INSDC first public

2014-11-20T17:01:17Z

INSDC last update

2018-03-08T15:56:47Z

INSDC status

public

StrainOrLine

C57BL/6

Submitter Id

E-MTAB-1265:mouse_testis_pol3_3

age

8 week

broker name

ArrayExpress

common name

house mouse

organism part

testis

sample name

E-MTAB-1265:mouse_testis_pol3_3

sex

male

Sequenced DNA Library

library_name

mouse_testis_pol3_3

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP sequencing experiments were performed as described previously (Schmidt, 2009) using the following antibodies H3K4me3 (Milipore, CMA304), H3K36me3 (abcam, ab9050), H3K27me3 (E1F) (Kimura et al), Pol I (sc28714), Pol II (abcam, ab5408), Pol III (1900) and MYBL1 (Sigma, HPA008791).

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

mm10

Number of total reads

17080376

Reads aligned (%)

89.8

Duplicates removed (%)

36.3

Number of peaks

674 (qval < 1E-05)

mm9

Number of total reads

17080376

Reads aligned (%)

89.7

Duplicates removed (%)

36.5

Number of peaks

740 (qval < 1E-05)