Illumina HiSeq 2500 sequencing; ChIP-seq of NuRD components and pluripotency factors in wild-type, Mbd3 and Sall4/1 mutant embryonic stem cells
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
No description
Antigen
NA
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
ENA first public
2016-08-09
ENA last update
2016-03-16
ENA-CHECKLIST
ERC000011
External Id
SAMEA3902986
INSDC center alias
University of Cambridge
INSDC center name
University of Cambridge
INSDC first public
2016-08-09T17:01:30Z
INSDC last update
2016-03-16T14:08:40Z
INSDC status
public
Submitter Id
E-MTAB-4565:Mbd3_Sall4null_2i_4
broker name
ArrayExpress
cell line
Sall4 KO
cell type
embryonic stem cell
common name
house mouse
genotype
Sall4-/-
sample name
E-MTAB-4565:Mbd3_Sall4null_2i_4
Sequenced DNA Library
library_name
Mbd3_Sall4null_2i_4
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ES cells were cultured on 0.1% gelatin-coated plates in 2i/LIF conditions. Cells were fixed using 1% formaldehyde for 10 min at room temperature and fixation quenched with 150 mM glycine. Chromatin was sheared to an average fragment size between 200 and 300 bp using a Bioruptor sonication instrument (Diagenode). Immunoprecipitation was performed using antibodies against endogenous proteins or the FLAG tag. Libraries were prepared with the NEXTflex Rapid Illumina DNA-seq Library Prep Kit (Bioo Scientific).