Illumina HiSeq 2000 sequencing; ChIP-sequencing of murine T-cell acute lymphoblastic leukemia (T-ALL) cell lines to investigate histone methylation profiling of lymphocyte deficient for Tet2 and mutated for DNMT3A
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
No description
Antigen
NA
Cell type
Cell type Class
Blood
Cell type
T-ALL
NA
NA
Attributes by original data submitter
Sample
ENA first public
2016-02-24
ENA last update
2015-12-23
ENA-CHECKLIST
ERC000011
External Id
SAMEA3719309
INSDC center alias
UMR 1170 INSERM Institut Gustave Roussy
INSDC center name
UMR 1170 INSERM Institut Gustave Roussy
INSDC first public
2016-02-24T17:02:28Z
INSDC last update
2015-12-23T14:11:04Z
INSDC status
public
Submitter Id
E-MTAB-4158:MO467_H3K4me3
broker name
ArrayExpress
cell line
mouse cell line
cell type
T cell
common name
house mouse
disease
T-cell acute lymphoblastic leukaemia
genotype
DNMT3A R882H Tet2-/-
sample name
E-MTAB-4158:MO467_H3K4me3
strain
C57BL/6
Sequenced DNA Library
library_name
MO467_H3K4me3
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cell lines were established after culture on MS5 feeder in MEMa medium supplemented with 10% FBS and 10ng/mL of murine IL7, FLT3L and SCF. MO467 cells were then grown in RPMI 1640 supplemented with 10% FBS and R152 with RPMI 1640 supplemented with 10% FBS and 10 ng/ml of murine IL7 SPRIworks (Beckman Coulter)