Illumina Genome Analyzer IIx sequencing; FOXM1 ChIP-seq in an OE33 cell line
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Digestive tract
Cell type
OE33
Primary Tissue
Esophagus
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
ENA checklist
ERC000011
INSDC center name
University of Manchester
INSDC first public
2015-12-17T13:48:37Z
INSDC last update
2015-12-14T11:44:54Z
INSDC status
public
SRA accession
ERS1016055
Sample Name
ERS1016055
alias
E-MTAB-4120:FOXM1 IP rep2
broker name
ArrayExpress
cell line
OE33
disease
oesophageal adenocarcinoma
disease state
oesophageal adenocarcinoma
organism
Homo sapiens
sex
female
title
FOXM1 IP rep2
Sequenced DNA Library
library_name
FOXM1 IP rep2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
OE33 cells were cultured in RPMI 1640 medium containing 2 mM glutamine and 10% Fetal Bovine Serum. After cross-link reversal and protein digestion with Proteinase K (Roche), DNA was purified using Qiaquick, PCR purification kit (Qiagen) following the manufacturers instructions. ChIP was performed as described in Lee et al. 2006 using 3x107 cells. Immunoprecipitation was performed overnight at 4C with 3 g antibody (anti-FOXM1 or control IgG) and 30 l Dynabeads per experiment. Lee, T. I., Johnstone, S. E., and Young, R. A. (2006). Chromatin immunoprecipitation and microarray-based analysis of protein location. Nat Protoc 1, 729748. doi:10.1038/nprot.2006.98.