Antigen

Antigen Class

No description

Antigen

NA

Cell type

Cell type Class

Liver

Cell type

Hepatocytes

MeSH Description

The main structural component of the LIVER. They are specialized EPITHELIAL CELLS that are organized into interconnected plates called lobules.

Sample

ENA first public

2012-06-22

ENA last update

2018-03-08

External Id

SAMEA1468566

INSDC center alias

CRUK-CRI

INSDC center name

CRUK-CRI

INSDC first public

2012-06-22T17:00:48Z

INSDC last update

2018-03-08T15:47:19Z

INSDC status

public

StrainOrLine

129/SvxC57BL/6

Submitter Id

E-MTAB-941b:Rad21+/- input 1

broker name

ArrayExpress

cell type

hepatocyte

common name

house mouse

organism part

liver

sample name

E-MTAB-941b:Rad21+/- input 1

Sequenced DNA Library

library_name

Rad21+/- input 1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Hepatocytes were prepared by direct perfusion of the liver with buffered salt solution followed by percent formaldehyde. After 10 minutes the tissue was removed and diced in 500 mM glycine buffer to neutralize the formaldehyde. After homogenization the hepatocytes were rinsed with PBS.

Sequencing Platform

instrument_model

Illumina Genome Analyzer

mm10

Number of total reads

42980606

Reads aligned (%)

98.0

Duplicates removed (%)

9.7

Number of peaks

763 (qval < 1E-05)

mm9

Number of total reads

42980606

Reads aligned (%)

97.9

Duplicates removed (%)

9.7

Number of peaks

858 (qval < 1E-05)