Illumina HiSeq 2000 paired end sequencing; Genome-wide distribution of histones modifications in mouse ESC Rif1+/+ and Rif1-/-
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
ENA checklist
ERC000011
INSDC center alias
EMBL
INSDC center name
European Molecular Biology Laboratory
INSDC first public
2015-12-24T17:01:47Z
INSDC last update
2018-03-09T02:14:56Z
INSDC status
public
SRA accession
ERS791008
Sample Name
ERS791008
alias
E-MTAB-3743:Rif1+/+1_input
broker name
ArrayExpress
cell line
ES cell line
genotype
wild type genotype
organism
Mus musculus
title
Rif1+/+1_input
Sequenced DNA Library
library_name
Rif1+/+1_input
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Inducible RsCre Rif1 ES cells have been cultured for two in presence of tamoxifen in order to delete Rif1. ChIP-seq libraries were prepared with NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (NEB#E6240S/L), except that bead purification was employed (Agencourt AMPure XP beads, Beckman Coulter A63881). Size selection was performed on 2% agarose gels (E-Gel Invitrogen G6610-02). Generally 13 PCR cycles for 10ng starting material were applied. Libraries were barcoded with the NEBNext Multiplex Oligos for Illumina (Index Primers Set 1, NEB#E7335S/L).