Antigen

Antigen Class

No description

Antigen

NA

Cell type

Cell type Class

Gonad

Cell type

Testis

MeSH Description

The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.

Sample

ENA first public

2011-07-29

ENA last update

2018-03-08

External Id

SAMEA787458

INSDC center alias

CRUK-CRI

INSDC center name

CRUK-CRI

INSDC first public

2011-07-29T17:00:39Z

INSDC last update

2018-03-08T15:27:40Z

INSDC status

public

StrainOrLine

C57BL/6J

Submitter Id

E-MTAB-424_complete:mmu0SQ121

broker name

ArrayExpress

common name

house mouse

developmental stage

adult

organism part

testes

sample name

E-MTAB-424_complete:mmu0SQ121

sex

male

Sequenced DNA Library

library_name

do1174_PolIII_testes_1900_mmu0SQ121

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Samples were prepared by direct perfusion of the tissue with buffered salt solution, followed by % formaldehyde. After 10 minutes, the tissue was removed and diced in 500 mM glycine buffer to neutralize the formaldehyde. After homogenization, the cells were rinsed with PBS. The animal tissues were crosslinked with formaldehyde treatment and chromatin fragmented to an average of 300 bp by sonication. Chromatin from an mass equivalent of 1/4 mouse liver was used for each ChIP experiment. Solexa libraries were prepared following the instructions of Illumina (Sample preparation for genomic DNA - version 2.2) with the following modifications. The ChIP-enriched DNA was not further fragmented. After end-repair and addition of an 'A' base to the 3' ends, the adapters were ligated to the ends of the DNA Fragments using 2 ul of fortyfold diluted 'Adapter oligo mix' in a total reaction volume of 25 ul. Between these steps, the DNA was purified using the DNA Clean-and-Concentrator-5 kit (Zymo Research). Subsequently, the DNA was amplified by 18 cycles of PCR, purified with QIAquick PCR purification Kit, and eluted with 33.5 ul of 10 mM tris buffer at pH7.0. The PCR-product was sized fractionated on 2% agarose gel and a gel slice containing the 200-300 bp fragments was excised. The flowcells were prepared and processed according to the manufacturer's protocols, with single-end sequencing for 36 to 45 cycles.

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

mm10

Number of total reads

33091860

Reads aligned (%)

91.6

Duplicates removed (%)

12.3

Number of peaks

1023 (qval < 1E-05)

mm9

Number of total reads

33091860

Reads aligned (%)

91.3

Duplicates removed (%)

12.3

Number of peaks

1218 (qval < 1E-05)