Illumina MiSeq paired end sequencing of SAMD00119012
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Yeast strain
Cell type
YTT121
NA
NA
Attributes by original data submitter
Sample
sample_name
UAF_ChIP004
genotype
MATa fob1Δ0 RDN1-150 his3Δ1 leu2Δ0 met15Δ0 ura3Δ0
mating_type
MATa
replicate
biological replicate 2
strain
YTT121
Sequenced DNA Library
library_name
WT_RDN1-150_IP2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
YPD cultured cells were cross-linked by 1 percent formaldehyde at 30degree for 30 minutes. After quenching with Glycine, the cell pellets of 21E9 cells were lysed by YTZ-beads. The extracts were recovered with 400 micro-l of lysis buffer 50 mM Hepes-KOH pH 7.5, 140 mM NaCl, 1 mM EDTA, 1 percent Triton-X100, 0.1 percent Na-deoxycholate., and sheared by Covaris S220 to 100bp average. After pelleting, volume of the soluble fraction was adjusted to 1115 micro-l. 50 microl of the extracts were used for INPUT samples. 10 micro-g of anti-HA F-7 mouse antibody was added to 1ml extract and immunoprecipitated with 100 micro-l of ProteinG-dyanabeads. After reverse-crosslinking and DNA purification, sequencing libraries were prepared by KAPA Hyperprep kit and TruseqHT-compatible adaptors. The ligated libraries were amplified by KAPA library amplification kit and purified by AMpureXP double size selection.