Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Gonad

Cell type

Testis

MeSH Description

The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.

Sample

sample_name

DRS013203

sample comment

The testes were crosslinked with 1% formaldehyde treatment and fixation was stopped by addition of 0.125 mM glycine. After washing with PBS, samples were stored at -80 C. The crosslinked material from 100 mg testes was sonicated to 500 bp fragments by Covaris S2 (duty cycle; 20 %, cycle per burst; 200, 10 times for 30 seconds). Immunoprecipitation was performed by incubatin overnight at 4 C with 2 ug of anti-H3K27me3 (Millipore 07-449), 2 ug of anti-H3K4me1 (abcam ab8895), 4 ug of anti-H3K4me3 (abcam ab1012), and control IgG. Antibody-protein complex was captured with dynabeads protein A or G beads (life technologies) and DNA was extracted.

Sequenced DNA Library

library_name

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library_strategy

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library_source

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library_selection

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library_construction_protocol

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Sequencing Platform

instrument_model

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cycle_sequence

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cycle_count

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flow_sequence

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flow_count

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key_sequence

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mm10

Number of total reads

21960712

Reads aligned (%)

42.7

Duplicates removed (%)

7.5

Number of peaks

678 (qval < 1E-05)

mm9

Number of total reads

21960712

Reads aligned (%)

42.6

Duplicates removed (%)

7.7

Number of peaks

715 (qval < 1E-05)