Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Gonad

Cell type

Testis

MeSH Description

The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.

Sample

sample_name

DRS013189

sample comment

The testes were crosslinked with 1% formaldehyde treatment and fixation was stopped by addition of 0.125 mM glycine. After washing with PBS, samples were stored at -80 C. The crosslinked material from 100 mg testes was sonicated to 500 bp fragments by Covaris S2 (duty cycle; 20 %, cycle per burst; 200, 10 times for 30 seconds). Immunoprecipitation was performed by incubatin overnight at 4 C with 2 ug of anti-H3K27me3 (Millipore 07-449), 2 ug of anti-H3K4me1 (abcam ab8895), 4 ug of anti-H3K4me3 (abcam ab1012), and control IgG. Antibody-protein complex was captured with dynabeads protein A or G beads (life technologies) and DNA was extracted.

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Library was constructed by using Illumina TruSeq ChIP Sample Prep Kit according manufacture's protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

23438247

Reads aligned (%)

92.3

Duplicates removed (%)

16.1

Number of peaks

16765 (qval < 1E-05)

mm9

Number of total reads

23438247

Reads aligned (%)

92.2

Duplicates removed (%)

16.2

Number of peaks

16798 (qval < 1E-05)