H3K27me3 ChIP IP sample (4 days after introduction of Ras)
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27me3
Cell type
Cell type Class
Embryonic fibroblast
Cell type
NIH/3T3
Primary Tissue
Embryo
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
sample_name
DRS011942
sample comment
For comprehensive analysis of Ras-dependent changes in H3K27me content, we performed ChIP-seq analyses at various times after Ras induction. We sampled NIH 3T3 cells at 0, 2, 4, 7, and 12 days after infection with the H-Ras(G12V) retroviral vector. ChIP-seq libraries were prepared from ~40 ng each of ChIP and input DNA with the use of a TruSeq DNA LT Sample Prep Kit (Illumina, San Diego, CA). Libraries were clonally amplified in a flow cell and sequenced with the use of HiSeq Control Software 1.5 (Illumina) and a 48-nucleotide paired-end sequence. Image analysis and base calling were performed with the use of Real Time Analysis (RTA) 1.13 software.